Article
- The EMBO Journal (2005) 24, 1775 - 1786
- doi:10.1038/sj.emboj.7600658
Published online: 5 May 2005
Subject Category:
Sec17p and HOPS, in distinct SNARE complexes, mediate SNARE complex disruption or assembly for fusion
Kevin M Collins1, Naomi L Thorngren1, Rutilio A Fratti1 and William T Wickner1
- Department of Biochemistry, Dartmouth Medical School, Hanover, NH, USA
Correspondence to:
William T Wickner, Department of Biochemistry, Dartmouth Medical School, 7200 Vail Building, Room 425 Remsen, Hanover, NH 03755-3844, USA. Tel.: +1 603 650 1701; Fax: +1 603 650 1353; Lab website: www.dartmouth.edu/~wickner/
Received 15 November 2004; Accepted 1 April 2005
Abstract
SNARE functions during membrane docking and fusion are regulated by Sec1/Munc18 (SM) chaperones and Rab/Ypt GTPase effectors. These functions for yeast vacuole fusion are combined in the six-subunit HOPS complex. HOPS facilitates Ypt7p nucleotide exchange, is a Ypt7p effector, and contains an SM protein. We have dissected the associations and requirements for HOPS, Ypt7p, and Sec17/18p during SNARE complex assembly. Vacuole SNARE complexes bind either Sec17p or the HOPS complex, but not both. Sec17p and its co-chaperone Sec18p disassemble SNARE complexes. Ypt7p regulates the reassembly of unpaired SNAREs with each other and with HOPS, forming HOPS
SNARE complexes prior to fusion. After HOPSSNARE assembly, lipid rearrangements are still required for vacuole content mixing. Thus, Sec17p and HOPS have mutually exclusive interactions with vacuole SNAREs to mediate disruption of SNARE complexes or their assembly for docking and fusion. Sec17p may displace HOPS from SNAREs to permit subsequent rounds of fusion.
Keywords:
- fusion,
- HOPS,
- membrane,
- Rab,
- SNARE
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