Article
- The EMBO Journal (2005) 24, 1863 - 1873
- doi:10.1038/sj.emboj.7600654
Published online: 5 May 2005
Subject Categories:
Impaired maturation of myeloid progenitors in mice lacking novel Polycomb group protein MBT-1
Satoko Arai1 and Toru Miyazaki1
- Center for Immunology, University of Texas Southwestern Medical Center, Dallas, TX, USA
Correspondence to:
Toru Miyazaki, Center for Immunology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard NA7200, Dallas, TX 75390-9093, USA. Tel.: +1 214 648 7322; Fax: +1 214 648 7331; E-mail: Toru.Miyazaki@UTSouthwestern.edu
Received 23 November 2004; Accepted 31 March 2005
Abstract
Polycomb group (PcG) proteins participate in DNA-binding complexes with gene-repressing activity, many of which have been highlighted for their involvement in hematopoiesis. We have identified a putative PcG protein, termed MBT-1, that is associated with Rnf2, an in vivo interactor of PcG proteins. MBT-1 structurally resembles the H-L(3)MBT protein, whose deletion is predicted to be responsible for myeloid hematopoietic malignancies. The human MBT-1 gene is located on chromosome 6q23, a region frequently deleted in leukemia cells, and shows a transient expression spike in response to maturation-inducing stimuli in myeloid leukemia cells. MBT-1-/- myeloid progenitor cells exhibit a maturational deficiency but maintain normal proliferative activities. This results in the accumulation of immature myeloid progenitors and hence, a marked decrease of mature myeloid blood cells, causing the MBT-1-/- mice to die of anemia during a late embryonic stage. Together, we conclude that MBT-1 specifically regulates the maturational advancement of myeloid progenitor cells during transitions between two developmental stages. We also show that MBT-1 appears to influence myelopoiesis by transiently enhancing p57KIP2 expression levels.
Keywords:
- cyclin-dependent kinase inhibitor (CDKI),
- hematopoiesis,
- mbt repeat,
- polycomb,
- Rnf2
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated
NEWS AND VIEWS
Hematopoiesis flies high with Ikaros
Nature Immunology News and Views (01 Apr 2006)
Nature News and Views (02 Jun 2005)
RESEARCH
Conditional MLL-CBP targets GMP and models therapy-related myeloproliferative disease
The EMBO Journal Article (26 Jan 2005)
Nature Immunology Article (01 Aug 2008)



