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| Subject Categories: Genome Stability & Dynamics |
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| The EMBO Journal
(2005) 24, 180–189, doi:10.1038/sj.emboj.7600485 Published online 25 November 2004 |
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| UvrD helicase, unlike Rep helicase, dismantles RecA nucleoprotein filaments in Escherichia coli |
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| Xavier Veaute1, 4, Stéphane Delmas2, 4, Marjorie Selva2, Josette Jeusset3, Eric Le Cam3, Ivan Matic2, Francis Fabre1 and Marie-Agnès Petit2, 5 |
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1 CEA, DSV, DRR, UMR217 CNRS/CEA, Fontenay aux roses, France 2 U571, INSERM, Faculté de Médecine Necker-Enfants, Malades, Paris, France 3 Interactions moléculaires et cancer, UMR 8126 CNRS/IGR/UPS, Institut Gustave Roussy, Villejuif, France 4 These two authors contributed equally to this work 5 Present address: URLGA, INRA, 78352 Jouy en Josas, France. Tel.: +33 1 34 65 20 64; Fax: +33 1 34 65 20 65 To whom correspondence should be addressed Xavier Veaute, CEA, INSERM, DRR, UMR217 CNRS/CEA, BP6, 92265 Fontenay aux roses, France. Tel.: +33 1 46 54 93 43; Fax: +33 1 46 54 95 98; E-mail: xavier.veaute@cea.fr Marie-Agnès Petit, CEA, INSERM, DRR, UMR217 CNRS/CEA, BP6, 92265 Fontenay aux roses, France. Tel.: +33 1 46 54 93 43; Fax: +33 1 46 54 95 98; E-mail: mapetit@jouy.inra.fr Received 27 August 2004; Accepted 27 October 2004; Published online 25 November 2004. |
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| Abstract |
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| The roles of UvrD and Rep DNA helicases of Escherichia coli are not yet fully understood. In particular, the reason for rep uvrD double mutant lethality remains obscure. We reported earlier that mutations in recF, recO or recR genes suppress the lethality of uvrD rep, and proposed that an essential activity common to UvrD and Rep is either to participate in the removal of toxic recombination intermediates or to favour the proper progression of replication. Here, we show that UvrD, but not Rep, directly prevents homologous recombination in vivo. In addition to RecFOR, we provide evidence that RecA contributes to toxicity in the rep uvrD mutant. In vitro, UvrD dismantles the RecA nucleoprotein filament, while Rep has only a marginal activity. We conclude that UvrD and Rep do not share a common activity that is essential in vivo: while Rep appears to act at the replication stage, UvrD plays a role of RecA nucleoprotein filament remover. This activity of UvrD is similar to that of the yeast Srs2 helicase. |
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| Keywords: helicase, RecA, RecF, Rep, UvrD |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated NEWS AND VIEWSGenetic recombination From competition to collaboration Nature News and Views (22 Jan 1998) Dual engines moving on antiparallel tracks Nature Structural Biology News and Views (01 Jul 2003) RESEARCHThe EMBO Journal Article (03 Feb 2003) UvrD controls the access of recombination proteins to blocked replication forks The EMBO Journal Article |
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