Figure 2
GADD45
/GADD45
and MEKK4 comprise a genetic pathway mediating STAT4-independent IFN production in T cells
Hongbo Chi, Binfeng Lu, Mutsuhiro Takekawa, Roger J Davis and Richard A Flavell
- The EMBO Journal (2004) 23, 1576 - 1586
- doi:10.1038/sj.emboj.7600173
Published online: 25 March 2004
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IFN
synthesis is reduced in differentiating Th1 cells from MEKK4-/- mice. (A) CD4 T cells were activated by anti-CD3, anti-CD28, anti-IL4, and different doses of IL12 for 4 days, and IFN levels were measured by ELISA. (B) CD4 T cells were activated as in (A) in the presence of 2 ng/ml IL12, and IFN levels were measured by ELISA after 3 and 6 days of stimulation. (C) Real-time PCR analysis of IFN mRNA was performed using RNA isolated from cells after 3 and 6 days of Th1 differentiation. The amount of IFN mRNA was normalized to that of HPRT mRNA. (D) CFSE-labeled CD4 T cells were activated under Th1 conditions for 3 days, followed by FACS analysis.
