Figure 1
GADD45
/GADD45
and MEKK4 comprise a genetic pathway mediating STAT4-independent IFN production in T cells
Hongbo Chi, Binfeng Lu, Mutsuhiro Takekawa, Roger J Davis and Richard A Flavell
- The EMBO Journal (2004) 23, 1576 - 1586
- doi:10.1038/sj.emboj.7600173
Published online: 25 March 2004
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Generation of MEKK4 mutant mice. (A) Targeting strategy. The targeting vector to replace the endogenous MEKK4 exon 3 sequence contains a lox site (indicated by the filled arrow) inserted upstream of MEKK4 exon 3, and a neomycin cassette (neo) flanked by two lox sites downstream of exon 3. Cre-mediated recombination in vivo resulted in the complete deletion of MEKK4 exon 3. XbaI* indicates that the restriction site was destroyed. (B) Southern blot analysis of genomic DNA from MEKK4+/+, +/- and -/- mice. DNA was digested with NcoI and hybridized to the 3' probe. The wild-type allele is 9.5 kb in length, and the mutant allele (with exon 3 deleted) is 7 kb. (C) Western blot analysis of activated CD4 T cells from MEKK4 +/+ and -/- mice. Two polyclonal antibodies that recognize the N-terminus (upper panel) and C-terminus (middle panel) of MTK1/MEKK4 coding sequence were used.
