Figure 4
MK2-induced tristetraprolin:14-3-3 complexes prevent stress granule association and ARE-mRNA decay
Georg Stoecklin, Tiffany Stubbs, Nancy Kedersha, Stephen Wax, William FC Rigby, T Keith Blackwell and Paul Anderson
- The EMBO Journal (2004) 23, 1313 - 1324
- doi:10.1038/sj.emboj.7600163
Published online: 11 March 2004
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Analysis of TTP truncation and substitution mutants. (A) Schematic representation of wild-type TTP as well as truncation and substitution mutants. NES: nuclear export signal; ZFD: zinc-finger domain; 14-3-3bs: 14-3-3-binding site. (B) Quantitative immunofluorescence analysis showing the percentage of transfected cells in which truncated TTP mutants accumulate in SGs (average values
s.e.m. of n
3 independent experiments). COS7 cells were transfected with TTP-myc, TTP-NZ-myc, TTP-Z-myc, TTP-ZC-myc and TTP-ZC-S178A-myc, and processed as described for Figure 3B. (C) Representative immunofluorescence micrographs of arsenite-treated COS7 cells transfected with TTP-ZC-myc or (D) TTP-ZC-S178A-myc. Left panels show the myc-tagged TTP mutants (red), center panels show TIA-1 as a marker of SGs (green, arrows) and right panels show merged views. Size bar is 10 
m.
