Article
- The EMBO Journal (2004) 23, 1267 - 1278
- doi:10.1038/sj.emboj.7600135
Published online: 18 March 2004
Subject Category:
Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi
Hidenori Hirose1,ab, Kohei Arasaki1,a, Naoshi Dohmae2, Koji Takio2, Kiyotaka Hatsuzawa1,c, Masami Nagahama1, Katsuko Tani1, Akitsugu Yamamoto3, Masaya Tohyama4 and Mitsuo Tagaya1
- School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan
- Biomolecular Characterization Division, RIKEN (The Institute of Physical and Chemical Research), Saitama, Japan
- Department of Cell Biology, Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga, Japan
- Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
Correspondence to:
Mitsuo Tagaya, School of Life Science, Tokyo University of Pharmacy and Life Science, Horinouchi Hachioji, Tokyo 192-0392, Japan. Tel.: +81 426 777496; Fax: +81 426 768866; E-mail: tagaya@ls.toyaku.ac.jp
aThese authors contributed equally to this work
bPresent address: Central Pharmaceutical Research Institute, Japan Tabaco Inc., Takatsuki, Osaka 569-1125, Japan
cPresent address: Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan
Received 27 June 2003; Accepted 20 January 2004
Abstract
ZW10, a dynamitin-interacting protein associated with kinetochores, is known to participate directly in turning off of the spindle checkpoint. In the present study, we show that ZW10 is located in the endoplasmic reticulum as well as in the cytosol during interphase, and forms a subcomplex with RINT-1 (Rad50-interacting protein) and p31 in a large complex comprising syntaxin 18, an endoplasmic reticulum-localized t-SNARE implicated in membrane trafficking. Like conventional syntaxin-binding proteins, ZW10, RINT-1 and p31 dissociated from syntaxin 18 upon Mg2+-ATP treatment in the presence of NSF and 
-SNAP, whereas the subcomplex was not disassembled. Overexpression, microinjection and knockdown experiments revealed that ZW10 is involved in membrane trafficking between the endoplasmic reticulum and Golgi. The present results disclose an unexpected role for a spindle checkpoint protein, ZW10, during interphase.
Keywords:
- endoplasmic reticulum,
- kinetochore,
- syntaxin 18,
- vesicular transport,
- ZW10
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