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The EMBO Journal (2004) 23, 959–968, doi:10.1038/sj.emboj.7600049 Published online 5 February 2004 |
| Supplementary information |
| An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's disease |
| Reiko Onuki, Yoshio Bando, Eigo Suyama, Taiichi Katayama, Hiroaki Kawasaki, Tadashi Baba, Masaya Tohyama and Kazunari Taira |
Supplementary Material 1 Control immunoblot for the fractionation of a nuclear-residents. Nuclear and cytoplasmic fractions were subjected to Western blotting with anti-histone and anti-actin antibodies as described in the text. Supplementary Material 2 Localization of WT PKR fused with GFP protein. GFP or WT PKR fused with GFP was transiently transfected into the SK-N-SH cells. Localization of GFP (A), GFP fused to N-terminus of PKR (B) and GFP fused to C-terminus of PKR was observed. Bar, 20 |
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