Article
- The EMBO Journal (2004) 23, 4868 - 4875
- doi:10.1038/sj.emboj.7600469
Published online: 18 November 2004
Subject Categories:
The CDK regulates repair of double-strand breaks by homologous recombination during the cell cycle
Yael Aylon, Batia Liefshitz and Martin Kupiec
- Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel
Correspondence to:
Martin Kupiec, Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 69978, Israel. Tel.: +972 3 640 9031; Fax: +972 3 640 9407; E-mail: martin@post.tau.ac.il
Received 19 August 2004; Accepted 12 October 2004
Abstract
DNA double-strand breaks (DSBs) are dangerous lesions that can lead to genomic instability and cell death. Eukaryotic cells repair DSBs either by nonhomologous end-joining (NHEJ) or by homologous recombination. We investigated the ability of yeast cells (Saccharomyces cerevisiae) to repair a single, chromosomal DSB by recombination at different stages of the cell cycle. We show that cells arrested at the G1 phase of the cell cycle restrict homologous recombination, but are able to repair the DSB by NHEJ. Furthermore, we demonstrate that recombination ability does not require duplicated chromatids or passage through S phase, and is controlled at the resection step by Clb–CDK activity.
Keywords:
- checkpoint,
- DNA repair,
- DNA replication,
- nonhomologous end-joining,
- yeast
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