Article
- The EMBO Journal (2004) 23, 4166 - 4176
- doi:10.1038/sj.emboj.7600427
Published online: 7 October 2004
Subject Categories:
TI-VAMP/VAMP7 is required for optimal phagocytosis of opsonised particles in macrophages
Virginie Braun1, Vincent Fraisier2, Graça Raposo3, Ilse Hurbain3, Jean-Baptiste Sibarita2, Philippe Chavrier1, Thierry Galli4 and Florence Niedergang1
- Membrane and Cytoskeleton Dynamics group, UMR144 CNRS-Institut Curie, Paris, France
- Digital Imaging Platform, UMR144 CNRS-Institut Curie, Paris, France
- Electron Microscopy Group, UMR144 CNRS-Institut Curie, Paris, France
- Membrane Traffic and Neuronal Plasticity, INSERM U536, Institut du Fer-à-Moulin, Paris, France
Correspondence to:
Florence Niedergang, UMR144 CNRS-Institut Curie, 26, rue d'Ulm, 75248 Paris cedex 05, France. Tel.: +33 1 42 34 63 67; Fax: +33 1 42 34 63 77; E-mail: florence.niedergang@curie.fr
Received 24 March 2004; Accepted 6 September 2004
Abstract
Phagocytosis relies on extension of plasmalemmal pseudopods generated by focal actin polymerisation and delivery of membranes from intracellular pools. Here we show that compartments of the late endocytic pathway, bearing the tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP/VAMP7), are recruited upon particle binding and undergo exocytosis before phagosome sealing in macrophages during Fc receptor (FcR)-mediated phagocytosis. Expression of the dominant-negative amino-terminal domain of TI-VAMP or depletion of TI-VAMP with small interfering RNAs inhibited phagocytosis mediated by Fc or complement receptors. In addition, inhibition of TI-VAMP activity led to a reduced exocytosis of late endocytic vesicles and this resulted in an early blockade of pseudopod extension, as observed by scanning electron microscopy. Therefore, TI-VAMP defines a new pathway of membrane delivery required for optimal FcR-mediated phagocytosis.
Keywords:
- endosomes,
- exocytosis,
- phagocytosis,
- recycling,
- SNARE
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