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The EMBO Journal (2004) 23, 4040–4050, doi:10.1038/sj.emboj.7600416 Published online 23 September 2004 Figure 4 Targeting of Swi/Snf to the yeast GAL1 UASG requires the Mediator, TAFIIs, and RNA polymerase II Karine Lemieux and Luc Gaudreau

Figure 4 Swi/Snf and SAGA are recruited to the GAL1 UASG by artificial recruitment of the Mediator. (A) Artificial recruitment of the Mediator bypasses the requirement for a transcriptional activator. A strain deleted for GAL4 and bearing a Myc-tagged version of Snf5 was transformed by a plasmid expressing the Gal4 DBD alone (KLY019) or fused to the C-terminal end of Gal11 (KLY018). Primer extension analyses were carried out with purified RNA from both strains. Yeast cells were grown in the presence of raffinose (-) and then galactose (+) was added in order to induce GAL1 gene expression. (B) ChIP analysis of the binding of Swi/Snf to the GAL1 UASG in yeast cells expressing Gal4-Gal11. Binding of Snf5-Myc in the strains used in (A). (C) Quantification of experiments as illustrated in (B) is shown. (D, E) Same experiment as in (B, D) with the exception that the binding of Gcn5-Myc (KLY044 and KLY043) was assessed. (F) ChIP analysis of the binding of Srb4-Myc and Rpb1 to the GAL1 UASG in yeast cells expressing a Gal4-Gal11 fusion (KLY091 and KLY092). (G) Quantification of experiments as illustrated in (F).

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