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Figure 5

MicroRNA genes are transcribed by RNA polymerase II

Yoontae Lee, Minju Kim, Jinju Han, Kyu-Hyun Yeom, Sanghyuk Lee, Sung Hee Baek and V Narry Kim

  • The EMBO Journal (2004) 23, 4051 - 4060
  • doi:10.1038/sj.emboj.7600385

Published online: 16 September 2004

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Analysis of the mir-23aapprox27aapprox24-2 gene promoter. (A) Schematic diagram of the reporter construct. The 5' end (X) of the promoter varies from -2010 to +1, while the 3' end corresponds to +36 relative to the transcription start site as determined in Figure 4. (B) Relative activities of luciferase expressed from the reporter constructs. The plasmids were cotransfected with Renilla luciferase construct into HEK293T cells and enzyme assay was carried out 48 h after transfection. Relative activities of the firefly luciferase are presented after normalization against the Renilla luciferase activity.

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