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| Subject Categories: RNA |
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| The EMBO Journal
(2004) 23, 376–385, doi:10.1038/sj.emboj.7600050 Published online 8 January 2004 |
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| Prp5 bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA |
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| Yong-Zhen Xu1, Catherine M Newnham1, Sei Kameoka2, Tao Huang1, Maria M Konarska2 and Charles C Query1 |
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1 Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY, USA 2 The Rockefeller University, New York, NY, USA To whom correspondence should be addressed Charles C Query, Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461-1975, USA. Tel.: +1 718 430 4174; Fax: +1 718 430 8574; E-mail: query@aecom.yu.edu Received 30 September 2003; Accepted 28 November 2003; Published online 8 January 2004. |
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| Abstract |
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| Communication between U1 and U2 snRNPs is critical during pre-spliceosome assembly; yet, direct connections have not been observed. To investigate this assembly step, we focused on Prp5, an RNA-dependent ATPase of the DExD/H family. We identified homologs of Saccharomyces cerevisiae Prp5 in humans (hPrp5) and Schizosaccharomyces pombe (SpPrp5), and investigated their interactions and function. Depletion and reconstitution of SpPrp5 from extracts demonstrate that ATP binding and hydrolysis by Prp5 are required for pre-spliceosome complex A formation. hPrp5 and SpPrp5 are each physically associated with both U1 and U2 snRNPs; Prp5 contains distinct U1- and U2-interacting domains that are required for pre-spliceosome assembly; and, we observe a Prp5-associated U1/U2 complex in S. pombe. Together, these data are consistent with Prp5 being a bridge between U1 and U2 snRNPs at the time of pre-spliceosome formation. |
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| Keywords: ATPase, spliceosome assembly, U1 snRNP, U2 snRNP, U1/U2 di-snRNP |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated NEWS AND VIEWSNature News and Views (07 Feb 1991) Nature News and Views (05 Jan 1989) RESEARCHPre-spliceosome formation in S.pombe requires a stable complex of SF1?U2AF 59 ?U2AF 23 The EMBO Journal Article (15 Oct 2002) Progression through the spliceosome cycle requires Prp38p function for U4/U6 snRNA dissociation The EMBO Journal Article (15 May 1998) |
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