Article
- The EMBO Journal (2004) 23, 3815 - 3824
- doi:10.1038/sj.emboj.7600400
Published online: 16 September 2004
Subject Category:
SWI/SNF remodeling and p300-dependent transcription of histone variant H2ABbd nucleosomal arrays
Dimitar Angelov1, André Verdel2, Woojin An3, Vladimir Bondarenko4, Fabienne Hans2, Cécile-Marie Doyen1,2, Vassily M Studitsky4, Ali Hamiche5, Robert G Roeder3, Philippe Bouvet1 and Stefan Dimitrov2
- Ecole Normale Supérieure de Lyon, CNRS-UMR 5161, Lyon, France
- Institut Albert Bonniot, INSERM U309, La Tronche, France
- Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY, USA
- Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Piscataway, NJ, USA
- Institut Andre Lwoff, CNRS UPR 9079, Villejuif, France
Correspondence to:
Philippe Bouvet, Ecole Normale Supérieure de Lyon, CNRS-UMR 5161, 46 Allée d'Italie, 69007 Lyon, France. Tel.: +33 472 72 80 16; Fax: +33 472 72 80 80; E-mail: pbouvet@ens-lyon.fr
Stefan Dimitrov, Laboratoire de Biologie Moléculaire, et Cellulaire de la Différenciation, Institut Albert Bonniot INSERM U309, Domaine de la Merci, 38706 La Tronche Cedex, France. Tel.: +33 476 54 94 73; Fax: +33 476 54 95 95; E-mail: stefan.dimitrov@ujf-grenoble.fr
Received 22 March 2004; Accepted 16 August 2004
Abstract
A histone variant H2ABbd was recently identified, but its function is totally unknown. Here we have studied the structural and functional properties of nucleosome and nucleosomal arrays reconstituted with this histone variant. We show that H2ABbd can replace the conventional H2A in the nucleosome, but this replacement results in alterations of the nucleosomal structure. The remodeling complexes SWI/SNF and ACF are unable to mobilize the variant H2ABbd nucleosome. However, SWI/SNF was able to increase restriction enzyme access to the variant nucleosome and assist the transfer of variant H2ABbd–H2B dimer to a tetrameric histone H3–H4 particle. In addition, the p300- and Gal4-VP16-activated transcription appeared to be more efficient for H2ABbd nucleosomal arrays than for conventional H2A arrays. The intriguing mechanisms by which H2ABbd affects both nucleosome remodeling and transcription are discussed.
Keywords:
- histone transfer,
- histone variant H2Abbd,
- nucleosome remodeling,
- transcription
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