Close window
The EMBO Journal (2004) 23, 3527–3537, doi:10.1038/sj.emboj.7600360 Published online 19 August 2004 Figure 6 BMP-2 decreases Mash1 stability by increasing Id1 expression Francesc Viñals, Julia Reiriz, Santiago Ambrosio, Ramon Bartrons, Jose Luis Rosa and Francesc Ventura

Figure 6 (A) E47 expression of DMS53 cell clones, stably overexpressing E47, was analyzed by immunoblotting and is shown in the upper panel. Parental and two independent clones (E10 and E17) were treated for 2 or 4 h with 5 nM BMP-2 and expressions of endogenous Mash1 and Id1 were analyzed by immunoblotting (lower panel). (B) Wild-type or Id1-/- MEFs were transfected with the indicated expression constructs. After 24 h, cells were splitted and treated with 10 nM BMP-2 for 3 h. Expressions of Mash1, Id1 and tubulin were analyzed by immunoblotting.

previous | next

Privacy policy	Copyright © 2004 by the European Molecular Biology Organization