The EMBO Journal
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The EMBO Journal (2004) 23, 3527–3537, doi:10.1038/sj.emboj.7600360

Published online 19 August 2004
Figure 4
BMP-2 decreases Mash1 stability by increasing Id1 expression
Francesc Viñals, Julia Reiriz, Santiago Ambrosio, Ramon Bartrons, Jose Luis Rosa and Francesc Ventura
Figure 4
Figure 4
(A, B) Extracts from HEK-293 cells transfected with the expression constructs indicated, treated or not with 50 muM LLnL for 8 h, were analyzed by immunoblotting. (C) HEK-293 cells, transfected with the constructs indicated, were pulsed with [35S]methionine for 3 h and labeled proteins were chased in unlabeled media for different times. Labeled Mash1 was immunoprecipitated and visualized by SDS–PAGE. Quantification of Mash1 levels from three to four separate assays is expressed as meanplusminuss.e.m. of percentage values of samples at time zero.
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