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The EMBO Journal (2004) 23, 3527–3537, doi:10.1038/sj.emboj.7600360 Published online 19 August 2004 Figure 2 BMP-2 decreases Mash1 stability by increasing Id1 expression Francesc Viñals, Julia Reiriz, Santiago Ambrosio, Ramon Bartrons, Jose Luis Rosa and Francesc Ventura

Figure 2 (A) Cell extracts were obtained from DMS53 cells treated with 5 nM BMP-2 for the times indicated and analyzed by immunoblotting. (B) Quantifications of Mash1 (filled circles) and Id1 (filled squares) protein levels from five separate assays were performed using -tubulin as normalization and were expressed as fold-change over control samples at time zero and expressed as means.e.m. (C) DMS53 cells were treated for 2 h with the indicated amounts of BMP-2 and Mash1 expression was analyzed by immunoblotting. (D) Parental and two independent myc-Mash1 overexpressing clones were treated for 2 h with 5 nM BMP-2 and expressions of ectopic Mash1 (upper panel) or endogenous Mash1 (lower panel) were analyzed by immunoblotting.

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