Close window
The EMBO Journal (2004) 23, 2577–2585, doi:10.1038/sj.emboj.7600268 Published online 10 June 2004 Figure 2 Negative feedback loop in T-cell activation through MAPK-catalyzed threonine phosphorylation of LAT Satoshi Matsuda, Yoshihiro Miwa, Yasuko Hirata, Akiko Minowa, Junko Tanaka, Eisuke Nishida and Shigeo Koyasu

Figure 2 Phosphorylation of LAT at Thr 155 by JNK and ERK. (A) GST-LAT proteins were incubated with active JNK (+) or inactive JNK (-) in the presence of [-32P]ATP at 30°C for 30 min. (B) GST-LAT proteins were phosphorylated with active JNK (lanes 2–5) or inactive JNK (lane 1), and subjected to immunoblot with an anti-phospho-Thr-Pro (Cell Signaling) antibody. (C) GST-LAT proteins were phosphorylated with active ERK, and subjected to immunoblot with the anti-phospho-Thr-Pro antibody. (D) GST-LAT or GST-ATF2 proteins were phosphorylated with active (+) or inactive (-) p38, and subjected to immunoblot with the anti-phospho-Thr-Pro antibody (upper) or anti-phospho-ATF2 antibody (lower). (E) LAT proteins were immunoprecipitated from transiently transfected COS7 cells, and subjected to immunoblot with the anti-phospho-Thr-Pro antibody or the antibody against LAT. (F) GFP-fused mouse LAT proteins were immunoprecipitated from transiently transfected Jurkat cells, which are cotransfected with (+) or without (-) SESE, and subjected to immunoblot with the anti-phospho-Thr-Pro antibody or the antibody against GFP.

previous | next

Privacy policy	Copyright © 2004 by the European Molecular Biology Organization