Article
- The EMBO Journal (2004) 23, 2336 - 2347
- doi:10.1038/sj.emboj.7600252
Published online: 27 May 2004
Subject Categories:
The ribosomal protein Rps15p is required for nuclear exit of the 40S subunit precursors in yeast
Isabelle Léger-Silvestre1,a, Philipp Milkereit1,ab, Sébastien Ferreira-Cerca1,b, Cosmin Saveanu2, Jean-Claude Rousselle3, Valérie Choesmel1, Cécile Guinefoleau1, Nicole Gas1 and Pierre-Emmanuel Gleizes1
- Laboratoire de Biologie Moléculaire Eucaryote, CNRS and Université Paul Sabatier, Toulouse, France
- Génétique des Interactions Macromoléculaires, Institut Pasteur, Paris, France
- Plateforme Protéomique, Institut Pasteur, Paris, France
Correspondence to:
Pierre-Emmanuel Gleizes, Laboratoire de Biologie Moléculaire Eucaryote (CNRS), 118 route de Narbonne, 31062 Toulouse cedex, France. Tel.: +33 561 33 59 26; Fax: +33 561 33 58 86; E-mail: gleizes@ibcg.biotoul.fr
aThese authors equally contributed to this work
bPresent address: Universität Regensburg, Universitätsstr. 31, 93053 Regensburg, Germany
Received 17 February 2004; Accepted 4 May 2004
Abstract
We have conducted a genetic screen in order to identify ribosomal proteins of Saccharomyces cerevisiae involved in nuclear export of the small subunit precursors. This has led us to distinguish Rps15p as a protein dispensable for maturation of the pre-40S particles, but whose assembly into the pre-ribosomes is a prerequisite to their nuclear exit. Upon depletion of Rps15p, 20S pre-rRNA is released from the nucleolus and retained in the nucleus, without alteration of the pre-rRNA early cleavages. In contrast, Rps18p, which contacts Rps15p in the small subunit, is required upstream for pre-rRNA processing at site A2. Most pre-40S specific factors are correctly associated with the intermediate particles accumulating in the nucleus upon Rps15p depletion, except the late-binding proteins Tsr1p and Rio2p. Here we show that these two proteins are dispensable for nuclear exit; instead, they participate in 20S pre-rRNA processing in the cytoplasm. We conclude that, during the final maturation steps in the nucleus, incorporation of the ribosomal protein Rps15p is specifically required to render the pre-40S particles competent for translocation to the cytoplasm.
Keywords:
- nuclear export,
- pre-ribosome,
- ribosomal protein,
- yeast
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated
REVIEWS
Exporting RNA from the nucleus to the cytoplasm
Nature Reviews Molecular Cell Biology Review (01 Oct 2007)
NEWS AND VIEWS
Nature News and Views (02 Dec 1967)
Cell biology: Organizing the nucleolus
Nature News and Views (05 Dec 1985)
RESEARCH
Nature Genetics Article (01 Sep 1999)
Heredity Original Article
