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| Subject Categories: Membranes & Transport | Proteins |
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| The EMBO Journal
(2004) 23, 45–53, doi:10.1038/sj.emboj.7600015 Published online 11 December 2003 |
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| The SNARE Ykt6 mediates protein palmitoylation during an early stage of homotypic vacuole fusion |
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| Lars EP Dietrich1, Rolf Gurezka1, 3, Michael Veit2 and Christian Ungermann1 |
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1 Biochemie-Zentrum Heidelberg (BZH), University of Heidelberg, Heidelberg, Germany 2 Department of Immunology and Molecular Biology, Vet.-Med. Faculty of the Free University Berlin, Berlin, Germany 3 Current address: BioReliance Manufacturing GmbH, Cernyring 22, 69115 Heidelberg, Germany To whom correspondence should be addressed Christian Ungermann, Biochemie-Zentrum Heidelberg (BZH), University of Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany. Tel.: +49 6221 544180; Fax: +49 6221 544366; E-mail: cu2@ix.urz.uni-heidelberg.de Received 25 August 2003; Accepted 17 October 2003; Published online 11 December 2003. |
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| Abstract |
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| The NSF homolog Sec18 initiates fusion of yeast vacuoles by disassembling cis-SNARE complexes during priming. Sec18 is also required for palmitoylation of the fusion factor Vac8, although the acylation machinery has not been identified. Here we show that the SNARE Ykt6 mediates Vac8 palmitoylation and acts during a novel subreaction of vacuole fusion. This subreaction is controlled by a Sec17-independent function of Sec18. Our data indicate that Ykt6 presents Pal-CoA via its N-terminal longin domain to Vac8, while transfer to Vac8's SH4 domain occurs spontaneously and not enzymatically. The conservation of Ykt6 and its localization to several organelles suggest that its acyltransferase activity may also be required in other intracellular fusion events. |
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| Keywords: palmitoylation, Sec18, vacuole fusion, Ykt6, SNARE |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated NEWS AND VIEWSNature News and Views (08 May 1997) Nature News and Views (25 Mar 1993) RESEARCHSub-microscopic Structure of Injured Surface of Paramecium cadatum Nature Letters to Editor (10 Oct 1964) The EMBO Journal Article (15 Jun 2001) |
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