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Article
Subject Categories: Cell Cycle | Genome Stability & Dynamics
The EMBO Journal (2003) 22, 1688–1696, doi:10.1093/emboj/cdg154
Rap1p telomere association is not required for mitotic stability of a C3TA2 telomere in yeast
Mary Kate Alexander and Virginia A. Zakian
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA

To whom correspondence should be addressed
Virginia A. Zakian, vzakian@molbio.princeton.edu

Received 7 November 2002; Revised 9 January 2003; Accepted 12 February 2003.
Abstract
Telomeric DNA usually consists of a repetitive sequence: C1–3A/TG1–3 in yeast, and C3TA2/T2AG3 in vertebrates. In yeast, the sequence-specific DNA- binding protein Rap1p is thought to be essential for telomere function. In a tlc1h mutant, the templating region of the telomerase RNA gene is altered so that telomerase adds the vertebrate telomere sequence instead of the yeast sequence to the chromosome end. A tlc1h strain has short but stable telomeres and no growth defect. We show here that Rap1p and the Rap1p-associated Rif2p did not bind to a telomere that contains purely vertebrate repeats, while the TG1–3 single-stranded DNA binding protein Cdc13p and the normally non-telomeric protein Tbf1p did bind this telomere. A chromosome with one entirely vertebrate-sequence telomere had a wild-type loss rate, and the telomere was maintained at a short but stable length. However, this telomere was unable to silence a telomere-adjacent URA3 gene, and the strain carrying this telomere had a severe defect in meiosis. We conclude that Rap1p localization to a C3TA2 telomere is not required for its essential mitotic functions.
Keywords: RAP1, RIF2, telomerase, telomere, yeast
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