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The EMBO Journal (2003) 22, 1370–1380, doi:10.1093/emboj/cdg121 Figure 2 The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes Thorsten Schäfer, Daniela Strau, Elisabeth Petfalski, David Tollervey and Ed Hurt

Figure 2 Sedimentation behavior of TAP-tagged proteins associated with 40S pre-ribosomes on sucrose density gradients. Ribosomal fractions (40S, 60S, 80S and polysomes) were determined by OD254nm measurement of the gradient fractions (upper graph), and the indicated TAP-tagged protein baits were visualized by western blot analysis of these gradient fractions using anti-protein A antibodies (lower panel).

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