The EMBO Journal
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The EMBO Journal (2003) 22, 324–334, doi:10.1093/emboj/cdg027

Figure 1
Hallmarks of homology recognition by RecA-like recombinases are exhibited by the unrelated Escherichia coli RecT protein
Philippe Noirot, Ravindra C. Gupta, Charles M. Radding and Richard D. Kolodner
Figure 1
Figure 1
 Aggregation of ssDNA but not dsDNA upon RecT binding. Reaction mixtures (30 mul) containing the indicated RecT concentrations were incubated in buffer T with 25 muM 32P-labeled M13mp19 linear dsDNA (lanes a–i) or with 12.5 muM 32P-labeled M13mp19 ssDNA (lanes j–r) for 20 min at 37°C. The reaction products were resolved by electrophoresis in a 0.6% agarose gel. Aggregated material remained at the gel origin. The positions of linear dsDNA and ssDNA are indicated by the arrows.
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