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| Subject Categories: Structural Biology | Genome Stability & Dynamics |
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| The EMBO Journal
(2003) 22, 3461–3471, doi:10.1093/emboj/cdg311 |
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| Structure of a trapped endonuclease III–DNA covalent intermediate |
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| J.Christopher Fromme1 and Gregory L. Verdine1, 2 |
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1 Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA 2 Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA To whom correspondence should be addressed Gregory L. Verdine, verdine@chemistry.harvard.edu Received 17 March 2003; Revised 1 May 2003; Accepted 1 May 2003. |
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| Abstract |
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| Nearly all cells express proteins that confer resistance to the mutagenic effects of oxidative DNA damage. The primary defense against the toxicity of oxidative nucleobase lesions in DNA is the base-excision repair (BER) pathway. Endonuclease III (EndoIII) is a [4Fe–4S] cluster-containing DNA glycosylase with repair activity specific for oxidized pyrimidine lesions in duplex DNA. We have determined the crystal structure of a trapped intermediate that represents EndoIII frozen in the act of repairing DNA. The structure of the protein–DNA complex provides insight into the ability of EndoIII to recognize and repair a diverse array of oxidatively damaged bases. This structure also suggests a rationale for the frequent occurrence in certain human cancers of a specific mutation in the related DNA repair protein MYH. |
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| Keywords: crystal structure, DNA glycosylase, DNA repair, endonuclease III, [4Fe–4S] cluster |
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