Figure 7
An essential role for Prox1 in the induction of the lymphatic endothelial cell phenotype
Jeffrey T. Wigle, Natasha Harvey, Michael Detmar, Irina Lagutina, Gerard Grosveld, Michael D. Gunn, David G. Jackson and Guillermo Oliver
- The EMBO Journal (2002) 21, 1505 - 1513
- doi:10.1093/emboj/21.7.1505
At E11.5, 
-gal-positive endothelial cells adopt a blood vascular phenotype in Prox1-null embryos. (A) Lymphatic endothelial cells (-Gal, green) of heterozygous embryos are double-labeled by LYVE-1 (red). Single-labeled LYVE-1 cells correspond to macrophages. (B) In contrast, in Prox1-null embryos, budding -gal-positive endothelial cells (green) do not express LYVE-1. (C) Lymphatic endothelial cells (-Gal, green) of heterozygous embryos express low levels of laminin (red). Blood vessels express high levels of laminin in their continuous basement membrane (arrowheads). (D) In contrast, -gal-positive endothelial cells (green) express high levels of laminin on their cell surfaces (red) in the Prox1-null littermates. (E) Similarly, the lymphatic endothelial cells (green) of the heterozygous embryos express very low levels of the blood vascular marker CD34 (red); however, the -gal-positive endothelial cells of Prox1 homozygous littermates (F) express high levels of CD34. (CV, cardinal vein). Scale bar: 100 
m.
