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  • The EMBO Journal (2002) 21, 1280 - 1288
  • doi:10.1093/emboj/21.6.1280



There is a Corrigendum (May 2002) associated with this Article.

The cell morphogenesis gene ANGUSTIFOLIA encodes a CtBP/BARS-like protein and is involved in the control of the microtubule cytoskeleton

U. Folkers1, V. Kirik2, U. Schöbinger1, S. Falk2, S. Krishnakumar3, M.A. Pollock3, D.G. Oppenheimer3, I. Day4, A.R. Reddy4, G. Jürgens1 and M. Hülskamp2

  1. ZMBP, Entwicklungsgenetik, Universität Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany
  2. University of Köln, Botanical Institute III, Gyrhofstrasse 15, D-50931 Köln, Germany
  3. Department of Biological Sciences, University of Alabama, 301 Biology, Tuscaloosa, AL 35487-0344, USA
  4. Department of Biology, Colorado State University, Fort Collins, CO 80526, USA

Correspondence to:

M. Hülskamp, E-mail: martin.huelskamp@uni-koeln.de

Received 29 August 2001; Accepted 30 January 2002; Revised 30 January 2002

The ANGUSTIFOLIA (AN) gene is required for leaf hair (trichome) branching and is also involved in polarized expansion underlying organ shape. Here we show that the AN gene encodes a C-terminal binding proteins/brefeldin A ADP-ribosylated substrates (CtBP/BARS) related protein. AN is expressed at low levels in all organs and the AN protein is localized in the cytoplasm. In an mutant trichomes, the organization of the actin cytoskeleton is normal but the distribution of microtubules is aberrant. A role of AN in the control of the microtubule cytoskeleton is further supported by the finding that AN genetically and physically interacts with ZWICHEL, a kinesin motor molecule involved in trichome branching. Our data suggest that CtBP/BARS-like protein function in plants is directly associated with the microtubule cytoskeleton.

  • Keywords:

    • Angustifolia,
    • Arabidopsis,
    • cell morphogenesis,
    • microtubules,
    • trichomes