Article
- The EMBO Journal (2002) 21, 5853 - 5863
- doi:10.1093/emboj/cdf585
Subject Category:
Assisted RNP assembly: SMN and PRMT5 complexes cooperate in the formation of spliceosomal UsnRNPs
Gunter Meister1 and Utz Fischer1
- Max-Planck Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany
Correspondence to:
Utz Fischer, E-mail: ufischer@biochem.mpg.de
Received 6 June 2002; Accepted 16 September 2002; Revised 16 September 2002
Abstract
Although spliceosomal Sm proteins can assemble spontaneously onto UsnRNA in vitro, this process requires assisting factors in vivo. SMN, the protein involved in spinal muscular atrophy, is part of a complex that contains the Sm proteins and serves as a critical factor for this reaction. Here, we have reconstituted the SMN-dependent assembly of UsnRNPs in vitro. We demonstrate that the SMN complex is necessary and sufficient for the assembly reaction. The PRMT5 complex, previously implicated in methylation and storage of Sm proteins, interacts with the SMN complex and enhances its activity in an ATP-dependent manner. These data uncover the SMN–PRMT5 complex as a functional entity that promotes the assisted assembly of spliceosomal UsnRNPs, and potentially other, RNA–protein complexes.
Keywords:
- PRMT5,
- RNP assembly,
- SMN,
- spliceosomes
