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Figure 7
Non-IR-targeted insertions. (A) Distribution of insertion sites in pAPT182. Genetic elements are indicated in the inner circles and include the following: gene encoding ampicillin resistance (bla), the ends IRR* and IRL*, the pBR322 origin of replication (Ori) and the reporter gene lacZ (see description of pAPT182, Figure 4A). The lines in the inner circle correspond to one orientation of insertion and those in the outer circle correspond to the opposite orientation. The numbers indicate the total number of insertion at a given site. The 'funnels' indicate IR-targeted insertions and numbers present the number of insertions found at a specific site. The left section shows the results obtained with OrfAB alone (0.42  M), while the right section shows those obtained in the presence of both OrfAB (0.42 M) and OrfA (0.7 and 7 M). (B) Alignment of target sites used by IS911. Forty-four target sites and the flanking DNA sequences were aligned as 88 half-sites. The analysis was performed on a 20 bp window flanking the duplicated insertion site. A table was generated by compiling the sum of each nucleotide at each position in a 20  4 table. The significance of the nucleotide distribution at a given site was determined by the  2 Pearson test [2 =  (o - c)2/c], where o and c are the observed and calculated values, respectively. It took into consideration the overall nucleotide composition of the target plasmid pAPT182 (22.59% A, 23% T, 27.28% G and 27.11% C) as calculated values. The positions at which a nucleotide bias occurs with >99% (P < 0.01) confidence levels are indicated by a '+'.
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