|
 |
|
|
| The EMBO Journal
(2001) 20, 1875–1887, doi:10.1093/emboj/20.8.1875 |
|
| The Arabidopsis thaliana ABC transporter AtMRP5 controls root development and stomata movement |
|
|
| Nicola Gaedeke1, 9, Markus Klein2, 9, Uener Kolukisaoglu3, Cyrille Forestier4, Axel Müller5, Mark Ansorge1, Dirk Becker6, Yasmine Mamnun7, Karl Kuchler7, Burkhard Schulz3, Bernd Mueller-Roeber1, 8 and Enrico Martinoia2 |
|
|
1 Max-Planck-Institut für Molekulare Pflanzenphysiologie (MPI-MP), D-14424 Potsdam, Germany 2 Institut de Botanique, Laboratoire de Physiologie Végétale, Université de Neuchâtel, Rue Emile Argand 13, CH-2007 Neuchâtel, Switzerland 3 Botanisches Institut II, Universität zu Köln, Max-Delbrück-Laboratorium in der Max-Planck-Gesellschaft, Carl-von-Linné-Weg 10, D-50829 Köln, Germany 4 CEA-CEN Cadarache, DEVM-LEMS, BP 1, F-13108 St Paul Lez Durance, France 5 Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, D-44780 Bochum, Germany 6 Institut für Botanik 1, Universität Würzburg, Julius-von-Sachs Platz 2, D-97082 Würzburg, Germany 7 Department of Molecular Genetics, University of Vienna, Dr Bohr-Gasse 9/2, A-1030 Vienna, Austria 8 Present address: Universität Potsdam, Institut für Biochemie und Biologie, Abteilung Molekularbiologie, Karl-Liebknecht-Strasse 25, Haus 20, D-14476 Golm/Potsdam, Germany 9 N.Gaedeke and M.Klein contributed equally to this work To whom correspondence should be addressed Enrico Martinoia, enrico.martinoia@bota.unine.ch Received 6 November 2000; Revised 16 February 2001; Accepted 20 February 2001. |
|
|
|
| Abstract |
|
In the present study, we investigated a new member of the ABC transporter superfamily of Arabidopsis thaliana, AtMRP5. AtMRP5 encodes a 167 kDa protein and exhibits low glutathione conjugate and glucuronide conjugate transport activity. Promotor– -glucuronidase fusion constructs showed that AtMRP5 is expressed mainly in the vascular bundle and in the epidermis, especially guard cells. Using reverse genetics, we identified a plant with a T-DNA insertion in AtMRP5 (mrp5-1). mrp5-1 exhibited decreased root growth and increased lateral root formation. Auxin levels in the roots of mrp5-1 plants were increased. This observation may indicate that AtMRP5 works as an auxin conjugate transporter or that mutant plants are affected in ion uptake, which may lead to changes in auxin concentrations. Experiments on epidermal strips showed that in contrast to wild type, the sulfonylurea glibenclamide had no effect on stomatal opening in mrp5-1 plants. This result strongly suggests that AtMRP5 may also function as an ion channel regulator. |
|
| Keywords: ABC transporter, Arabidopsis, AtMRP5, root development, stomata |
|
|
| |
| |
| |
 | Email link to a friend |
| |
 | Download PDF |
| |
 | Full text |
| |
| |
| |
 | Next article |
| |
 | Previous article |
| |
 | Table of contents |
| |
| |
 | Rights and permissions |
| |
 | Reprints |
| |
| |
| |
 Register for table of contents by email | |
| |
| |
| |
