Figure 2
A role for histone deacetylase HDAC1 in modulating the transcriptional activity of MyoD: inhibition of the myogenic program
Asoke Mal, Michael Sturniolo, R. Louis Schiltz, Mrinal K. Ghosh and Marian L. Harter
- The EMBO Journal (2001) 20, 1739 - 1753
- doi:10.1093/emboj/20.7.1739
The association between P/CAF and MyoD occurs only in differentiated cells. (A) Western analysis of immunoprecipitates of MyoD obtained from extracts of proliferating C2 cells and C2 cells cultured in DM for the indicated times. The probe was anti-P/CAF. The lower panel represents the level of MyoD in extracts of C2 cells cultured in DM for the indicated times, as judged by western analysis with the use of anti-MyoD. An anti-GAPDH antibody was also used to ensure equal loading. (B) The level of P/CAF in extracts of proliferating cells (GM) or cells cultured in DM for the indicated times was analyzed by western blotting using anti-P/CAF antibody as probe. The membrane was also probed with anti-GAPDH to monitor equal loading of the extracts.
