Supplementary information
Adenovirus-activated PKA and p38/MAPK pathways boost microtubule-mediated nuclear targeting of virus
M. Suomalainen, M.Y. Nakano, K. Boucke, S. Keller and U.F. Greber
- The EMBO Journal (2001) 20, 1310 - 1319
- doi:10.1093/emboj/20.6.1310
Supplementary figure 7. PKA and p38 are not required for Ad2 internalization and delivery to the cytosol. (A) The kinetics of viral uptake into HeLa cells treated with the PKA inhibitor KT5720 or the p38-inhibitor SB203580 was determined using [35S]methionine-labeled Ad2 and a cell surface trypsinization assay that measured the emergence of a trypsin-resistant form of the major capsid protein hexon. The fraction of trypsin-resistant hexon was plotted as a function of infection time. There is no difference between the inhibitor-treated and the control cells. (B) Quantitative EM analyses of viral particles in PKI-myr-, SB- and no drug-treated cells. There are no differences in the amounts of virus particles at the plasma membrane, in intracellular vesicles and in the cytosol at 30 min post-infection (p >0.1)
Movie 1Aa. Time-lapse microscopy of microinjected Ad2-TR in HeLa cells. 11 min post-injection..
Movie 1Ab. Time-lapse microscopy of microinjected Ad2-TR in HeLa cells. 22 min post-injection.
Movie 1B. Time-lapse microscopy of microinjected Ad2-TR in HeLa cells. Challenge with wild-type Ad2 infection, 20 min post-injection.
Movie 1C. Time-lapse microscopy of microinjected Ad2-TR in HeLa cells. Challenge with wild-type Ad2 infection, nocodazole (20 
M), 22 min post-injection.
Movie 6A. Time-lapse microscopy of HeLa cell infections with Ad2-TR. No drug, 25 min post-injection.
Movie 6B. Time-lapse microscopy of HeLa cell infections with Ad2-TR. Nocodazole (20 M), 38 min post-injection.
Movie 6C. Time-lapse microscopy of HeLa cell infections with Ad2-TR. PKI-myr (55 M), 27 min post-injection.
Movie 6D. Time-lapse microscopy of HeLa cell infections with Ad2-TR. PKI-myr (55 M), nocodazole (20 M), 32 min post-injection.
Movie 6E. Time-lapse microscopy of HeLa cell infections with Ad2-TR. SB203580 (10 M), 28 min post-injection.
Movie 6F. Time-lapse microscopy of HeLa cell infections with Ad2-TR. SB203580 (10 M), nocodazole (20 M), 37 min post-injection.
Movie 6G. Time-lapse microscopy of HeLa cell infections with Ad2-TR. SB203580 (10 M), PKI-myr (55 M), 31 min post-injection.
Movie 6H. Time-lapse microscopy of HeLa cell infections with Ad2-TR. No drug, 5 min post-injection (cell surface).
Movie 6I. Time-lapse microscopy of MK2 wild-type MEF infections with Ad2-TR. No drug, 37 min post-injection.
Movie 6J. Time-lapse microscopy of MK2-deficient MEF infections with Ad2-TR. No drug, 26 min post-injection.
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