Figure 1
Signalling via vascular endothelial growth factor receptor-3 is sufficient for lymphangiogenesis in transgenic mice
Tanja Veikkola, Lotta Jussila, Taija Makinen, Terhi Karpanen, Michael Jeltsch, Tatiana V. Petrova, Hajime Kubo, Gavin Thurston, Donald M. McDonald, Marc G. Achen, Steven A. Stacker and Kari Alitalo
- The EMBO Journal (2001) 20, 1223 - 1231
- doi:10.1093/emboj/20.6.1223
Receptor specificity and expression of the transgene-encoded proteins. (A) Receptor binding analysis of metabolically labelled VEGF-C, VEGF-D and VEGF-C156S using soluble human or mouse VEGFR-2 and VEGFR-3. (B) Immunoprecipitation and phosphotyrosine analysis of VEGFR-2 and VEGFR-3 after stimulation of primary dermal microvascular endothelial cells with starvation medium, VEGF-D or VEGF-C156S. The asterisk denotes an intracellular VEGFR-3 precursor, which is not phosphorylated upon stimulation. (C) Transgene mRNA expression in K14-VEGF-D and K14-VEGF-C156S mice, and the levels of VEGFR-2 and VEGFR-3 mRNA were determined by northern blotting and hybridization of total skin RNA with the specific probes. WT, wild type; TG, transgenic mouse.
