Article
- The EMBO Journal (2001) 20, 6946 - 6957
- doi:10.1093/emboj/20.24.6946
Prospore membrane formation linked to the leading edge protein (LEP) coat assembly
Alexandra C. Moreno-Borchart1,2, Katrin Strasser1,2, Martin G Finkbeiner1,2, Anna Shevchenko3,4, Andrej Shevchenko3,4 and Michael Knop1,2
- Abteilung Molekulare Zellbiologie, Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, D-82152 Martinsried, Germany
- Present address: EMBL, Cell Biology and Biophysics, Meyerhofstrasse 1, D-69117 Heidelberg, Germany
- Protein and Peptide Group, EMBL, Meyerhofstrasse 1, D-69117 Heidelberg, Germany
- Present address: MPI for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany
Correspondence to:
Michael Knop, E-mail: michael.knop@embl-heidelberg.de
Received 26 September 2001; Accepted 1 November 2001; Revised 25 October 2001
Abstract
In yeast, the differentiation process at the end of meiosis generates four daughter cells inside the boundaries of the mother cell. A meiosis-specific plaque (MP) at the spindle pole bodies (SPBs) serves as the starting site for the formation of the prospore membranes (PSMs) that are destined to encapsulate the post-meiotic nuclei. Here we report the identification of Ady3p and Ssp1p, which are functional components of the leading edge protein (LEP) coat, that covers the ring-shaped opening of the PSMs. Ssp1p is required for the assembly of the LEP coat, which consists of at least three proteins (Ssp1p, Ady3p and Don1p). The assembly of the LEP coat starts with the formation of cytosolic precursors, which then bind in an Ady3p-dependent manner to the SPBs. Subsequent processes at the SPBs leading to functional LEP coats require Ssp1p and the MP components. During growth of the PSMs, the LEP coat functions in formation of the cup-shaped membrane structure that is indispensable for the regulated cellularization of the cytoplasm around the post-meiotic nuclei.
Keywords:
- Ady3p,
- meiosis,
- membrane organization,
- spindle pole body,
- Ssp1p
