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  • The EMBO Journal (2001) 20, 5049 - 5059
  • doi:10.1093/emboj/20.18.5049

Identification and functional reconstitution of the yeast peroxisomal adenine nucleotide transporter

Luigi Palmieri1,4, Hanspeter Rottensteiner2,4, Wolfgang Girzalsky2,3, Pasquale Scarcia1, Ferdinando Palmieri1 and Ralf Erdmann2

  1. Department of Pharmaco-Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, Via E. Orabona 4, 70125 Bari, Italy
  2. Institute of Chemistry/Biochemistry, Free University of Berlin, Thielallee 63, 14195 Berlin, Germany
  3. Present address: Institute of Physiological Chemistry, Ruhr-University Bochum, 44780 Bochum, Germany
  4. L.Palmieri and H.Rottensteiner contributed equally to this work

Correspondence to:

Ralf Erdmann, E-mail: ralferdm@zedat.fu-berlin.de

Received 21 May 2001; Accepted 31 July 2001; Revised 19 July 2001

The requirement for small molecule transport systems across the peroxisomal membrane has previously been postulated, but not directly proven. Here we report the identification and functional reconstitution of Ant1p (Ypr128cp), a peroxisomal transporter in the yeast Saccharomyces cerevisiae, which has the characteristic sequence features of the mitochondrial carrier family. Ant1p was found to be an integral protein of the peroxisomal membrane and expression of ANT1 was oleic acid inducible. Targeting of Ant1p to peroxisomes was dependent on Pex3p and Pex19p, two peroxins specifically required for peroxisomal membrane protein insertion. Ant1p was essential for growth on medium-chain fatty acids as the sole carbon source. Upon reconstitution of the overexpressed and purified protein into liposomes, specific transport of adenine nucleotides could be demonstrated. Remarkably, both the substrate and inhibitor specificity differed from those of the mitochondrial ADP/ATP transporter. The physiological role of Ant1p in S.cerevisiae is probably to transport cytoplasmic ATP into the peroxisomal lumen in exchange for AMP generated in the activation of fatty acids.

  • Keywords:

    • adenine nucleotide transporter,
    • mitochondrial carrier family,
    • peroxisomes,
    • Saccharomyces cerevisiae,
    • YPR128c