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Figure 3
Structure of Tetrahymena G-strand overhangs. (A) Identity of the terminal nucleotide. 3' end-labeled DNA was cleaved at DMS-modified G-residues: lane 1, control oligonucleotide 5' (T2G4)4; lane 2, control oligonucleotide 5'-G(T2G4)3T; lanes 3 and 4, rDNA from two different Tetrahymena clones; lane 5, Euplotes DNA. (B) Overhang length at rDNA and non-rDNA telomeres: lane 1, input guide oligonucleotide TG6; lane 2, marker oligonucleotide showing the expected size of the input oligonucleotide + 9 nt extension product; lanes 3–11, primer extension products obtained with T4 DNA polymerase; lane 12, products obtained with Sequenase; lanes 3 and 4, total DNA from exponentially growing and starved cells; lanes 5 and 6, non-rDNA from growing and starved cells; lanes 7 and 8, rDNA from growing and starved cells; lanes 9–12, rDNA from growing cells; lane 9, control reaction without ligase; lanes 10 and 11, Exo1 and T4 polymerase digestion prior to ligation. The overhang lengths corresponding to specific reaction products are indicated on the right.
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