Article
- The EMBO Journal (2001) 20, 3518 - 3525
- doi:10.1093/emboj/20.13.3518
An upstream repressor element plays a role in Igf2 imprinting
Sharon Eden1, Miguel Constancia2, Tamar Hashimshony1, Wendy Dean2, Beth Goldstein1, Alfred C. Johnson3, Ilana Keshet1, Wolf Reik2 and Howard Cedar1
- Department of Cellular Biochemistry, Hebrew University, Ein Kerem, Jerusalem 91120, Israel
- Programme in Developmental Genetics, The Babraham Institute, Cambridge CB2 4AT, UK
- Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
Correspondence to:
Howard Cedar, E-mail: cedar@md2.huji.ac.il
Received 20 April 2001; Accepted 11 May 2001; Revised 10 May 2001
Abstract
The imprinted Igf2 gene is associated with a small upstream region that is differentially methylated on the active paternal allele. We have identified a repressor element within this sequence and shown that repression is probably mediated through a trans- acting factor, GCF2. DNA methylation of this site abrogates both protein binding and repressor activity. Targeting experiments demonstrate that this element plays a role in the repression of the maternal Igf2 gene in vivo.
Keywords:
- GCF,
- imprinting,
- methylation
