Table 3
Signal transduction between a membrane-bound transporter, PtsG, and a soluble transcription factor, Mlc, of Escherichia coli
Sung-Jae Lee, Winfried Boos, Jean-Pierre Bouché and Jacqueline Plumbridge
- The EMBO Journal (2000) 19, 5353 - 5361
- doi:10.1093/emboj/19.20.5353
| Plasmid | ptsG-lacZ | |
|---|---|---|
| Gly | Glc | |
JM-G96 ( | ||
| pTZ(PtsG) | 9.5 | 39 |
pACH (IICGlcHis6) and pTZ(PtsG 5–320) | 12.5 | 33 |
| pACH (IICGlcHis6) and pJBH (IIBGlcHis6) | 9.5 | 11 |
| pACH (IICGlcHis6) | 15 | – |
ptsG, ptsM8) was transformed with the plasmids indicated and 
-galactosidase activities measured during growth in minimal medium with glycerol (0.4%) or glucose (0.2%), 0.1 mM IPTG, 0.5 mg/ml ampicillin for cultures with pTZ(PtsG), pTZ(PtsG
g/ml kanamycin for cultures with pACH. Results are the mean of three independent cultures.