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The EMBO Journal (2000) 19, 3990–4003, doi:10.1093/emboj/19.15.3990 Figure 10 Skin and hair follicle integrity is crucially dependent on 1 integrin expression on keratinocytes Cord Brakebusch, Richard Grose, Fabio Quondamatteo, Angel Ramirez, Jose L. Jorcano, Alison Pirro, Marcus Svensson, Rainer Herken, Takako Sasaki, Rupert Timpl, Sabine Werner and Reinhard Fässler

Figure 10 Infiltration of inflammatory cells in the mutant skin and RNase protection assay. Back skin sections of 16-day-old control (A and C) and mutant (B and D) mice were stained for F4/80 (macrophage/monocyte specific antigen; A, B) and Gr-1 (granulocyte specific antigen; C, D). In mutant skin, an infiltration of macrophages and granulocytes was observed. Some deformed hair follicles were surrounded by macrophages (arrows in B). (Staining of the sebaceous glands in (C) (arrow) and (D) is background staining; bar, 100 m.) (E) RNA isolated from skin of 4-week-old mutant and normal mice was analyzed by RNase protection (for details see Materials and methods). Mutant skin (fl/fl cre) showed a strong upregulation of IL-1 mRNA compared with littermate controls (control) (1000 c.p.m.: radiolabeled RNA probe; tRNA: negative control). Gel separated RNA samples were stained with ethidium bromide (EtBr) to control RNA amounts used in the assay.

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