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The EMBO Journal (2000) 19, 3990–4003, doi:10.1093/emboj/19.15.3990 Figure 1 Skin and hair follicle integrity is crucially dependent on 1 integrin expression on keratinocytes Cord Brakebusch, Richard Grose, Fabio Quondamatteo, Angel Ramirez, Jose L. Jorcano, Alison Pirro, Marcus Svensson, Rainer Herken, Takako Sasaki, Rupert Timpl, Sabine Werner and Reinhard Fässler

Figure 1 Generation of mice with a keratinocyte-restricted ablation of the 1 integrin gene. (A) Schematic presentation of the floxed 1 integrin gene and the cre-mediated deletion of the gene. After deletion of the 1 integrin gene, the 1 integrin promoter will transcribe the lacZ cDNA. (E, exons in arbitrary numbering; D, splice variant; pA, polyadenylation sequence of 1 integrin gene). Epidermis of back skin of 9-day-old control (B and C) and mutant mice (D and E) stained for 1 integrin (B and D) and lacZ activity (C and E) (bar, 50 m). Note the loss of 1 integrin expression in the mutant basal keratinocytes (arrows in B and D). Bulb region of hair follicles of back skin of 9-day-old control (F and H) and mutant mice (G and I) double-stained for 1 (red) and 6 integrin (green) (F and G) and for lacZ activity (H and I) (bar, 50 m). Note the loss of 6 and 1 integrin in the hair matrix and the thin unstained region visible between the 6 integrin expressing ORS cells and 1 integrin expressing cells surrounding the hair follicle (G). Control (J) and mutant (K) mice at 4 weeks of age. Mutant mice were approximately half the weight of control littermates, lost nearly all hair, and had frequent wounds, especially in mechanically stressed regions of the skin.

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