Definitive hematopoietic stem cells first develop within the major arterial regions of the mouse embryo

The EMBO Journal (2000) 19, 2465–2474, doi:10.1093/emboj/19.11.2465

Table 1

Marella F.T.R. de Bruijn, Nancy A. Speck, Marian C.E. Peeters and Elaine Dzierzak

Table 1: HSC activity in directly transplanted and cultured E11 and E12 aorta and UGR

		No. of mice repopulated/total transplanted^a

Tissue	Embryo equivalent	E11	E12	E11 cultured^b	E12 cultured^b
Aorta^c	1	7/17	17/29	17/28	6/6
	0.3	4/15	1/6	8/20	0/6
UGR	4.3^d	0/3
	1	0/10	5/26	18/26	2/6
	0.3	0/9	0/4	8/19	2/5
AGM	1	7/8	7/9	9/11	4/5
	0.3	1/6	1/2	5/11	4/5

^aOnly mice with 10% donor repopulation in the peripheral blood at 4–9 months after transfer were included as positive. Data are from a total of 21 independent experiments. ^bOrgan explants were cultured for 3 days prior to transplantation. ^cAorta region: includes surrounding mesenchyme. ^dAn average of 4.3 e.e. were transplanted in two experiments (range 4–5).



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