Figure 5
Conformation of the Bax C-terminus regulates subcellular location and cell death
Amotz Nechushtan, Carolyn L. Smith, Yi-Te Hsu and Richard J. Youle
- The EMBO Journal (1999) 18, 2330 - 2341
- doi:10.1093/emboj/18.9.2330
Different GFP–Bax mutants display opposite effects on cell viability. (A) L929 mouse fibroblasts, and (B) and (C) Bax-/- cells were transiently co-transfected with the pGL3 luciferase reporter construct (0.25 
g) and different GFP fusion constructs of either wild-type or mutated Bax (1 g). The C3-EGFP expression vector was transfected as a control. After 40 h of incubation, cells were harvested and processed for luciferase assays. Results were quantitated with a scintillation counter and displayed as a percentage of the control. The error bars show a mean standard error determined from at least three independent measurements.
