Figure 1
Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity
Natalia Levina, Sabine Tötemeyer, Neil R. Stokes, Petra Louis, Michael A. Jones and Ian R. Booth
- The EMBO Journal (1999) 18, 1730 - 1737
- doi:10.1093/emboj/18.7.1730
Hydrophobicity plot for KefA and YggB. The hydrophobicity plots were constructed by standard methods (Kyte and Doolittle, 1982) using a window of 19 residues in the DNASTAR suite of programmes. On the basis of sequence homologies and alkaline phosphatase fusion data, the KefA protein is predicted to consist of up to five domains (G.Gouesbet, M.A.Jones, N.R.Stokes, D.McLaggan, W.Epstein and I.R.Booth, manuscript in preparation). An N-terminal signal sequence is followed by a hydrophilic domain that is either periplasmic or inserted in the outer membrane, two inner membrane domains and a C-terminal cytoplasmic domain. The YggB protein shows greatest homology to the second transmembrane domain and the C-terminal hydrophilic domain.
