Figure 2
Calreticulin functions in vitro as a molecular chaperone for both glycosylated and non-glycosylated proteins
Yoshiro Saito, Yoshito Ihara, Michael R. Leach, Myrna F. Cohen-Doyle and David B. Williams
- The EMBO Journal (1999) 18, 6718 - 6729
- doi:10.1093/emboj/18.23.6718
(A and B) Effect of CRT on the thermal aggregation of non-glycosylated proteins. CS or MDH dimers (1 
M) were incubated at 45°C in the absence or presence of various concentrations of CRT (0.125–2 M) or IgG (1 or 2 M) as indicated. Aggregation was monitored by measuring light scattering at 360 nm. (C) Effect of ERp57 on the aggregation-suppressing function of CRT. Various combinations of MDH (0.6 M), CRT (0.24 M) and ERp57 (0.24 M) were incubated at 45°C as indicated and aggregation was monitored. In one case (designated 'pre'), CRT and ERp57 were pre-incubated at 25°C for 30 min and then incubated with MDH at 45°C.
