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The EMBO Journal (1999) 18, 5061–5072, doi:10.1093/emboj/18.18.5061 Figure 4 The E6 protein of human papillomavirus type 16 binds to and inhibits co-activation by CBP and p300 Daksha Patel, Shih-Min Huang, Laurel A. Baglia and Dennis J. McCance

Figure 4 Interaction of different p300 domains with E6.16 mutants. Radiolabeled in vitro translated wild-type E6.16, E6.6 and the indicated E6.16 mutants proteins were incubated with either (A) GST–C/H3, (B) GST–C/H1 or (C) GST–CT3 and –CT4 fusion proteins. The bound complexes were removed with glutathione beads, separated on a 15% SDS–polyacrylamide gel and quantified using a PhosphorImager and Imagequant software (Molecular Dynamics). See Table I. The input is 10% of that added to the test reactions. The Coomassie Blue-stained gel of the GST–CT3 and –CT4 fusion proteins used in (C) is shown below the gel.

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