| Table 1: Strains and plasmids used in this study |
 |
| Strain/plasmid |
Relevant genotype or structures |
Source or reference |
| Strains |
|
| TP2811 |
(= R1330) F- xyl argH1  lacX74 aroB ilvA . ptsH ptsI crr)::neo |
Levy et al. (1990) |
| W3110 |
(= R1128) F-  - IN(rrnD-rrnE)1 |
Bachmann (1972) |
| R1279 |
(pho-bgl)201 . lac-pro) ara thi rpsL |
Schnetz et al. (1996) |
| R1653 |
as R1279 but (ptsH ptsI crr)::neo |
R1279 T4(TP2811); Krieg (1993) |
| R1752 |
as R1279 but attB::bla Ptac bglG bglF |
R1279pFDX3410; this work |
| R2013 |
as R1752 but (ptsH ptsI crr)::neo |
R1752T4(TP2811); this work |
| R1958 |
as R1279 but attB::bla Ptac bglG |
R1279pFDX3157; this work |
| R2051 |
as R1958 but (ptsH ptsI crr)::neo |
R1958T4(TP2811); this work |
| R1967 |
as R1279 but (fruB fruK fruA) |
R1279pFDX3212; this work |
| R1971 |
as R1967 but attB:: bla Ptac bglG bglF |
R1967pFDX3410; this work |
| R1977 |
as R1971 but (ptsH ptsI crr)::neo |
R1971T4(TP2811); this work |
| R1974 |
as R1967 but attB::bla Ptac bglG |
R1967pFDX3157; this work |
| R1979 |
as R1974 but (ptsH ptsI crr)::neo |
R1974T4(TP2811); this work |
| Plasmids |
|
| pFDY226 |
lacIq P16 bglt2 lacZ (bla; oriR pBR322) |
Schnetz et al. (1996) |
| pFDY225 |
as pFDY226 but lacking the bglt2 |
Schnetz et al. (1996) |
| pFDX2676 |
as pFDY226 but bla replaced by tet |
Krieg (1993) |
| pFDX3158 |
as pFDY226 but bla replaced by cat |
this work |
| pFDX2469 |
bglG under control of Ptac (tet; oriR pACYC177) |
this work |
| pFDX3549 |
as pFDX2469 but bglG replaced by lacZ |
this work |
| pLDR8 |
int under control of PR, cI857 (neo; oriR pSC101; repTS) |
Diederich et al. (1992) |
| pFDX3401 |
as pLDR8 but neo replaced by cat |
this work |
| pLDR10 |
multiple cloning site; attP (bla; cat; oriR pBR322) |
Diederich et al. (1992) |
| pFDX3410 |
pLDR10 carrying Ptac bglG bglF within multiple cloning site |
this work |
| pFDX3157 |
pLDR10 carrying Ptac bglG within multiple cloning site |
this work |
| pFDX3155 |
ptsH ptsI under control of Ptac (cat; oriR pACYC177) |
this work |
| pFDX3160 |
ptsH under control of Ptac (tet; oriR pACYC177) |
this work |
| pFDX3161 |
ptsI under control of Ptac (tet; oriR pACYC177) |
this work |
| pFDX3212 |
flanking regions of fru chromosomal region (1kb resp.) (tet; oriR pSC101; repTS) |
this work |
| pFDX3223 |
ptsHH15A ptsI under control of Ptac (tet; oriR pACYC177) |
this work |
| pFDX3221 |
fruB ptsI under control of Ptac (tet; oriR pACYC177) |
this work |
| pFDX3102 |
Ptac bglG bglF; SD sequence of phage T7gene10 preceding bglG (tet; oriR pACYC177) |
this work |
| pFDX2942 |
as pFDX3102 but bglF removed |
this work |
| pFDY45 |
lacIq Ptac bglG; SD T7gene10 preceding bglG (bla; oriR pBR322) |
Schnetz and Rak (1990) |
| pFDX3225 |
as pFDX2942 but galK bglG |
this work |
| pFDX3226 |
as pFDX3102 but galKbglG |
this work |
| pFDX3293 |
as pFDX3102 but bglF replaced by nagE |
this work |
| pFDX3295 |
as pFDX3102 but bglF replaced by mtlA |
this work |
| pFDX3283 |
as pFDY226 but additionally carrying bglF under control of Ptac |
this work |
| pFDX3297 |
nagE under control of Ptac (neo; oriR pACYC177) |
this work |
| pFDX3298 |
mtlA under control of Ptac (neo; oriR pACYC177) |
this work |
| pFDX3299 |
bglF under control of Ptac (neo; oriR pACYC177) |
this work |
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