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| The EMBO Journal
(1998) 17, 6827–6838, doi:10.1093/emboj/17.23.6827 |
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Crystal structure of Escherichia coli cystathionine  -synthase at 1.5 Å resolution |
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| Tim Clausen, Robert Huber, Lars Prade, Markus C. Wahl and Albrecht Messerschmidt |
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Max-Planck-Institut für Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, 82152 Martinsried, Germany To whom correspondence should be addressed Tim Clausen, clausen@biochem.mpg.de Received 18 August 1998; Revised 6 October 1998; Accepted 7 October 1998. |
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| Abstract |
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The transsulfuration enzyme cystathionine -synthase (CGS) catalyses the pyridoxal 5'-phosphate (PLP)-dependent -replacement of O-succinyl-L-homoserine and L-cysteine, yielding L-cystathionine. The crystal structure of the Escherichia coli enzyme has been solved by molecular replacement with the known structure of cystathionine  -lyase (CBL), and refined at 1.5 Å resolution to a crystallographic R-factor of 20.0%. The enzyme crystallizes as an  4 tetramer with the subunits related by non-crystallographic 222 symmetry. The spatial fold of the subunits, with three functionally distinct domains and their quarternary arrangement, is similar to that of CBL. Previously proposed reaction mechanisms for CGS can be checked against the structural model, allowing interpretation of the catalytic and substrate-binding functions of individual active site residues. Enzyme–substrate models pinpoint specific residues responsible for the substrate specificity, in agreement with structural comparisons with CBL. Both steric and electrostatic designs of the active site seem to achieve proper substrate selection and productive orientation. Amino acid sequence and structural alignments of CGS and CBL suggest that differences in the substrate-binding characteristics are responsible for the different reaction chemistries. Because CGS catalyses the only known PLP-dependent replacement reaction at C of certain amino acids, the results will help in our understanding of the chemical versatility of PLP. |
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| Keywords: methionine biosynthesis, molecular modelling, protein structure, pyridoxal 5'-phosphate, X-ray crystallography |
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