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The EMBO Journal (1998) 17, 335–343, doi:10.1093/emboj/17.2.335 Figure 6 Electron microscopic structure of agrin and mapping of its binding site in laminin-1 Alain J. Denzer, Therese Schulthess, Charlotte Fauser, Beat Schumacher, Richard A. Kammerer, Jürgen Engel and Markus A. Ruegg

Figure 6 The N-terminal agrin fragment binds to the central region of the long arm of laminin-1. Electron micrographs of rotary-shadowed laminin-1, the C8-9 and E8 laminin fragments and the N-terminal agrin fragment cN25Fc. (A) Recombinant cN25Fc (open arrowheads) binds to a single site in the central region of the long arm of laminin-1. No globular particle is seen in rotary-shadowed laminin-1 (B) while recombinant cN25Fc appears after rotary shadowing (C) either as a globular particle or as a globule from which two globular units emerge (asterisks). (D) The C8-9 fragment of laminin-1 (arrow) frequently is associated with cN25Fc (open arrowheads). (E) No association of cN25Fc is observed with the E8 fragment (arrows). Bar: 100 nm.

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