Close window
The EMBO Journal (1998) 17, 335–343, doi:10.1093/emboj/17.2.335 Figure 5 Electron microscopic structure of agrin and mapping of its binding site in laminin-1 Alain J. Denzer, Therese Schulthess, Charlotte Fauser, Beat Schumacher, Richard A. Kammerer, Jürgen Engel and Markus A. Ruegg

Figure 5 Binding of agrin to the laminin C8-9 fragment is of high affinity. Dose–response curves for the binding of iodinated cAgrin to laminin-1 and to the C8-9 fragment. The binding curve shown is the result of one representative experiment. Each data point represents the mean SD of three measurements with the corresponding background counts (bovine serum albumin-coated wells) subtracted. The Kd values for the binding to laminin-1 and to C8-9 in this particular experiment were 2.0 and 1.6 nM, respectively. Note that the Kd values varied between independent dose–response curves (Kd values: laminin-1, 6.5–2.0 nM; C8-9 fragment, 4.2–1.6 nM).

previous | next

Privacy policy	Copyright © 1998 by the European Molecular Biology Organization