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The EMBO Journal (1998) 17, 4511–4526, doi:10.1093/emboj/17.15.4511 |
| Figure 6 |
| Protein domains and conformational changes in the activation of RepA, a DNA replication initiator |
| Rafael Giraldo, José M. Andreu and Ramón Díaz-Orejas |
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| Privacy policy | Copyright © 1998 by the European Molecular Biology Organization |
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N37. Protein samples in storage buffer were run at 0.4 ml/min through a Superose-12 HR-10/30 column at room temperature. Elution profiles were obtained monitoring A280 of the eluate. Insets to the panels indicate the sample identity, the concentration at injection and the species assigned to the observed peaks: monomers (wide and irregular), dimers (narrow and sharp) or larger aggregates. The estimated Rs and a cartoon with the proposed shapes (compact and spherical versus flexible and extended) for the proteins in the main peaks are also shown (colour coding for the domains is as in Figure 9).