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The EMBO Journal (1998) 17, 4491–4502, doi:10.1093/emboj/17.15.4491 Figure 7 Importin , transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells Stefan Jäkel and Dirk Görlich

Figure 7 Transportin has distinct binding sites for the M9 domain and the L23a interaction domain. Binding of transportin or importin to residues 32–74 of rpL23a (BIB domain) was performed as described in Figure 4. Note that the addition of 5 M of the GST–M9 fusion did not compete the rpL23a–transportin interaction. Instead, GST–M9 was recovered in the bound fraction, indicating the formation of a trimeric L23a–transportin–M9 complex. As a control, GST–M9 was not bound if transportin was replaced by importin (right panel). The asterisk denotes 2z–BIB fusion protein that had leaked from the column.

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